BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer
The BeNano Series represents the latest advancements in nanoparticle size and zeta potential analysis, engineered by Bettersize Instruments. The system integrates Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), and Static Light Scattering (SLS) to deliver precise measurements of particle size, zeta potential, and molecular weight. The BeNano Series finds extensive use in both academic research and industrial manufacturing across diverse fields, including chemical engineering, pharmaceuticals, food and beverage, inks and pigments, life science, and more.
Features and Advantages of BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer
- The size range of the BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer is: 0.3 nm - 15 μm.
- The minimum sample volume of the BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer is: 3 μL.
- The BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer features an APD (Avalanche Photodiode) detector for exceptional sensitivity.
- BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer has automatic laser intensity adjustment.
- BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer has an intelligent result evaluation algorithm.
- The BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer features DLS backscatter (173°) detection.
- The BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer has a user adjustable scattering volume for concentrated samples.
- BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer has PALS (Phase Analysis Light Scattering) technology.
- The BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer has programmable temperature control.
- BeNano 180 Zeta Pro Nanoparticle Size and Zeta Potential Analyzer complies with 21 CFR Part 11, ISO 22412, ISO 13099.
Features
Unlocking Research Potential with BeNano
Advanced ELS Technology: PALS
PALS technology excels at distinguishing and extracting electrophoretic behavior, even in samples with weak electrophoretic mobilities, whether near the isoelectric point or in high-salinity environments.
Advanced DLS Technology: Backscattering Detection
Backscattering DLS optics capture a significantly larger scattering volume compared to 90-degree optics. When coupled with a movable measurement position, backscattering DLS delivers heightened sensitivity and the capacity to measure samples with high turbidity.
Temperature Trend Measurement
For thermally sensitive samples, a temperature trend can be effortlessly executed using a programmed SOP. The BeNano can identify the temperature transition point in the size results, which corresponds to the aggregation temperature for protein samples.
Stable and Durable Optical Bench
The BeNano employs a 50mW solid-state laser, a single-mode fiber system, and a high-performance APD detector, ensuring stable, wide-ranging, and highly redundant detection capabilities.
Research-Grade Software
The BeNano software intelligently evaluates and processes scattered light signals to enhance signal quality and result stability. A variety of built-in calculation modes cater to diverse scientific research and application needs.
Ultra-Low Sample Volume Requirement
Measuring trace amounts of samples is crucial for early-stage R&D in the pharmaceutical industry and academia. The capillary sizing cell enables precise size measurements with just 3 to 5 μL of sample.
1. Particle Size Measurement via Dynamic Light Scattering (DLS)
Dynamic Light Scattering (DLS), also known as Photon Correlation Spectroscopy (PCS) or Quasi-Elastic Light Scattering (QELS), measures Brownian motion within a dispersant. It operates on the principle that smaller particles exhibit faster movement compared to larger particles. An avalanche photodiode (APD) detects the scattering intensities of the particles, which a correlator then converts into a correlation function. A mathematical algorithm applied to this correlation function yields the diffusion coefficient (D). The Stokes-Einstein equation, which establishes a relationship between the diffusion coefficient and particle size, enables the calculation of the hydrodynamic diameter (DH) and its distribution.
2. Backscattering Detection Technology
Features
- Wider Concentration Range: By strategically optimizing the detection position, highly concentrated samples can be analyzed near the sample cell's edge, effectively minimizing the multiple light scattering effect.
- Higher Sensitivity: Achieves 8-10 times the scattering volume and approximately 10 times the sensitivity compared to traditional 90° optical designs.
- Higher Size Upper Limit: This technology mitigates multiple light scattering from large particles and, to some extent, reduces number fluctuations of large particles due to the significantly larger scattering volume.
- Better Reproducibility: DLS backscattering technology is less susceptible to dust contaminants and unevenly distributed agglomerates, resulting in improved reproducibility.
Intelligent Search for Optimal Detection Position
The software autonomously determines the ideal detection position based on the sample's size, concentration, and scattering ability. This ensures the highest measurement accuracy and offers flexibility in analyzing samples with diverse properties and concentrations.
(1) Detection point in the middle of the sample cell: This results in a large scattering volume, enhancing instrument sensitivity and making it suitable for analyzing dilute samples with weaker scattering effects.
(2) Detection point at the edge of the sample cell: This avoids the multiple scattering effect in high-concentration samples, guaranteeing accurate and repeatable particle size results.
3. DLS Flow Mode
DLS flow mode delivers high-resolution size results for complex, polydisperse systems. When integrated with front-end separation equipment like GPC/SEC or FFF, particles are separated into monodisperse fractions and sequentially flow through the BeNano based on size. The size of each fraction is continuously measured and combined to generate a high-resolution size distribution.
BeNano can acquire RI or UV signals, providing more accurate volume and number distributions independent of algorithms compared to batch-mode measurements.
Applications
- Characterizing particulates and polymers based on size and dispersity
- Distinguishing monomers, dimers, and aggregates of proteins
- Effluent curves of size, intensity, and refractive index (RI)
4. High-resolution Size Measurement
Features
- DLS analyzer connects with GPC/SEC, FFF, etc.
- Receives up to 3 signals from RI, UV, or other detectors
- 27uL low-volume flow cell to prevent band broadening
- Size resolution as high as 1.3 : 1
- Size distributions weighted by number and volume, in addition to intensity
- Suitable for complex, polydisperse systems like proteins, polymers, etc.
5. Zeta Potential Measurement via Electrophoretic Light Scattering (ELS)
In aqueous systems, charged particles are enveloped by counterions, forming an inner Stern layer and an outer shear layer. Zeta potential represents the electrical potential at the shear layer interface. A higher zeta potential signifies greater stability and reduced aggregation within the suspension system. Electrophoretic Light Scattering (ELS) measures electrophoretic mobility through Doppler shifts in scattered light, which can then be used to determine the zeta potential of a sample using Henry's equation.
6. Phase Analysis Light Scattering (PALS)
Phase Analysis Light Scattering (PALS) is an advanced technology built upon traditional ELS, further developed by Bettersize for measuring zeta potential and its distribution within a sample.
Features and Benefits
- Accurate measurement of samples with low electrophoretic mobility
- Effective for samples in organic solvents with low dielectric constants
- More precise results for samples with high conductivity
- Effectively measures the zeta potential of particles whose charge approaches the isoelectric point
7. Static Light Scattering
Static Light Scattering (SLS) is a technology that measures scattering intensities, weight-average molecular weight (Mw), and the second virial coefficient (A2) of a sample using the Rayleigh equation
8. Microrheology Measured by DLS
Dynamic Light Scattering Microrheology (DLS Microrheology) is a cost-effective and efficient technique employing dynamic light scattering to ascertain rheological properties. By analyzing the Brownian motion of colloidal tracer particles, information about the system's viscoelastic properties, such as viscoelastic modulus, complex viscosity, and creep compliance, can be obtained using the generalized Stokes-Einstein equation.
Features & Benefits
- Investigates rheological behaviors by measuring the thermally-driven motion of tracer particles within the studied material
- Facilitates measurement across a broad range of frequencies
- Applies low stress to tracer particles
- Requires only microliter-scale sample volume
- Complements mechanical rheology results
- Suitable for weakly structured samples
9. Temperature Trend Measurement
Measurement Parameters
- Size vs. Temperature
- Zeta Potential vs. Temperature
Features
- Benefits protein formulation stability studies
- Accelerates real-time aging through elevated temperature simulation
Benefits
- Easy examination of protein formulation stability
- Accelerates real-time aging through elevated temperature simulation
10. pH Trend Measurement
Measurement Parameters
- Zeta Potential vs. pH
- Isoelectric point
- Conductivity vs. pH
Features
- High-precision ternary titration pumps
- Controllable peristaltic pump with high flow capacity and rate
- General-purpose electrode
- Automated titrant selection based on initial and target pH using intelligent software
Benefits
- Completes measurements in less time
- Improves consistency and repeatability of results
- Reduces researcher workload
- Simplifies operator qualifications
- Accelerates real-time aging through elevated temperature simulation
- Reduces exposure to corrosive liquids
